Expérimental
The project will aim at better understanding the molecular events that govern the architecture and dynamics of actin networks. In particular, using in vitro approaches (microfluidics, micropatterning and fluorescence microscopy), specific actin networks with specific mechanical constraints will be built and their labeling by different fluorescent probes will be assessed. Those results will be correlated to in vivo observations in a developmental context. This internship will be carried out in close collaboration between the two labs. The experiments will allow us to discover how actin probes can specifically label specific actin networks in cells and propose new views on the internal dynamics and their protein content. This internship will be an excellent opportunity to be placed at the interface of two fields of biology by discovering in vitro experiments with purified proteins and the benefit of great control of experimental conditions and characterizing intracellular dynamics in a developing whole organism (in vivo).