Expérimental
Super-resolution (SR) microscopy have revolutionized our understanding of the biological processes at the molecular level. Imaging single molecules and localizing their center with high precision allows the reconstruction of pointillist image of the sample with a resolution beyond the diffraction limit [1,2,3]. In order to capture the 3D nanoscale morphology of the whole cell, multifocus microscopy (MFM) has been proposed to instantaneously acquire the 3D localization of single molecules (SM) in cells within a volume of a few micrometers [4,5]. Still additional information such as molecular orientation can provide supplementary information relating the local molecular organization and arrangement to the biological function. One strategy to recover the orientation of single molecules is by polarimetric measurement [6]. A polarization-splitting scheme combined with SMLM allows to determine both the 2D localization and orientation of SM. The aim of this internship is to retrieve the orientation measurement simultaneously with 3D positional information of single molecules in biological samples. The method will rely on combining MFM with polarization measurements at the different focal plane.