Expérimental
We have developed a highly sensitive, easy-to-use, and versatile detection method using luminescent YVO4:Eu nanoparticles as probes, enabling the detection of both proteins and nucleic acids. Our method uses a protocol similar to that of existing immunoassays and is 20 to 1,000 times more sensitive than existing methods. The aim of this internship is to elucidate why and how the gain in sensitivity achieved by our method depends on the target molecule to be detected and, in particular, on the molecular interactions with the recognition molecules.