This project proposes to characterize intracellular rheology of epithelial cells under compressive stress. The aim is to understand the link between rheological properties, cellular activity and energy production in cells, and how these intracellular physical parameters are modified under compressive stresses, both in vitro and in integrated models (living tissues). The long-term goal is to uncover how mechanical compression could promote cell apoptosis through a modulation of intracellular rheological properties, both in vitro and in physiological integrated models. This project relies on the characterization of cellular crowding signature using particle tracking in living cells submitted to controlled compressive stress.